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staphylococcus aureus mssa atcc 25923 strain  (ATCC)


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    ATCC staphylococcus aureus mssa atcc 25923 strain
    Staphylococcus Aureus Mssa Atcc 25923 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 34630 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 34630 article reviews
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    Incubation:

    Article Title: Genetic and Phenotypic Characteristics of Five Staphylococcus aureus Strains Isolated from Yakutian Cattle.
    Article Snippet: Antibiotic susceptibility testing was performed using the disc diffusion method for the following antibiotics (HiMedia Laboratories Pvt., Ltd.) on Mueller–Hinton Agar (HiMedia Laboratories Pvt., Ltd.): penicillin, 10 iu; cefoxitin, 30 mkg; ciprofloxacin, 5 mkg; gentamicin, 10 mkg; kanamycin, 30 mkg; erythromycin, 15 mkg; clindamycin, 2 mkg; lincomycin, 15 mkg; rifampicin, 5 mkg; flucidin, 10 mkg; and tetraciclin, 30 mkg. .. The dishes with inoculated cultures were incubated under aerobic conditions at 37 ± 1 ◦C for 18–24 h. The Staphylococcus aureus (MSSA) ATCC 25923 strain, obtained from the collection of the State Scientific Center for Applied Microbiology and Biotechnology (SSC AMB, Moscow, Russia), was used as a positive control; Staphylococcus aureus (MRSA) NCTC 12493 (BD Diagnostics, Franklin Lakes, NJ, USA) was used as a negative control. .. Sterile saline solution (PanEco, Moscow, Russia) was used as a negative control.

    Positive Control:

    Article Title: Genetic and Phenotypic Characteristics of Five Staphylococcus aureus Strains Isolated from Yakutian Cattle.
    Article Snippet: Antibiotic susceptibility testing was performed using the disc diffusion method for the following antibiotics (HiMedia Laboratories Pvt., Ltd.) on Mueller–Hinton Agar (HiMedia Laboratories Pvt., Ltd.): penicillin, 10 iu; cefoxitin, 30 mkg; ciprofloxacin, 5 mkg; gentamicin, 10 mkg; kanamycin, 30 mkg; erythromycin, 15 mkg; clindamycin, 2 mkg; lincomycin, 15 mkg; rifampicin, 5 mkg; flucidin, 10 mkg; and tetraciclin, 30 mkg. .. The dishes with inoculated cultures were incubated under aerobic conditions at 37 ± 1 ◦C for 18–24 h. The Staphylococcus aureus (MSSA) ATCC 25923 strain, obtained from the collection of the State Scientific Center for Applied Microbiology and Biotechnology (SSC AMB, Moscow, Russia), was used as a positive control; Staphylococcus aureus (MRSA) NCTC 12493 (BD Diagnostics, Franklin Lakes, NJ, USA) was used as a negative control. .. Sterile saline solution (PanEco, Moscow, Russia) was used as a negative control.

    Negative Control:

    Article Title: Genetic and Phenotypic Characteristics of Five Staphylococcus aureus Strains Isolated from Yakutian Cattle.
    Article Snippet: Antibiotic susceptibility testing was performed using the disc diffusion method for the following antibiotics (HiMedia Laboratories Pvt., Ltd.) on Mueller–Hinton Agar (HiMedia Laboratories Pvt., Ltd.): penicillin, 10 iu; cefoxitin, 30 mkg; ciprofloxacin, 5 mkg; gentamicin, 10 mkg; kanamycin, 30 mkg; erythromycin, 15 mkg; clindamycin, 2 mkg; lincomycin, 15 mkg; rifampicin, 5 mkg; flucidin, 10 mkg; and tetraciclin, 30 mkg. .. The dishes with inoculated cultures were incubated under aerobic conditions at 37 ± 1 ◦C for 18–24 h. The Staphylococcus aureus (MSSA) ATCC 25923 strain, obtained from the collection of the State Scientific Center for Applied Microbiology and Biotechnology (SSC AMB, Moscow, Russia), was used as a positive control; Staphylococcus aureus (MRSA) NCTC 12493 (BD Diagnostics, Franklin Lakes, NJ, USA) was used as a negative control. .. Sterile saline solution (PanEco, Moscow, Russia) was used as a negative control.



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    A single-nucleotide polymorphism-based phylogenetic tree of methicillin resistance-lacking mec (MRLM) strains of Staphylococcus aureus . The whole genome sequences of all 13 strains analysed in this study (in red) were examined alongside those of 150 published MRLM strains, , downloaded from the NCBI database. The following MSSA genome sequences were also included: seven reference strains <t>ATCC</t> <t>25923</t> <t>(ST243/CC30,</t> <t>CP009361.1),</t> ATCC 29213 (ST5, GCA_001267715.2), ATCC 12600 (ST8, GCA_006094915.1), ATCC 9144 (ST30, GCA_900457695.1), NCTC 8325 (ST8, GCA_000013425.1), MSSA476 (ST1, GCA_000011525.1) and MSSA6850 (ST50, GCA_000462955.1), and eight previously reported clinical MSSA strains 10-00598 (ST22, GCA_028076695.1), 15-02736 (ST291, GCA_028082935.1), 16-01080 (ST45, GCA_028080235.1), 16-02665 (ST25, GCA_028075835.1), 18-01695 (ST398, GCA_028081975.1), 18-01787 (ST5, GCA_028081295.1), 19-00515 (ST15, GCA_028081935.1), and C00012754 (ST20, ERR418969). , Sequence types (STs) (inner ring), clonal complexes (CCs) (middle ring) and spa types (outer ring) are annotated. The tree scale indicates the number of substitutions per genome.
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    mssa strain atcc 25923  (ATCC)
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    A single-nucleotide polymorphism-based phylogenetic tree of methicillin resistance-lacking mec (MRLM) strains of Staphylococcus aureus . The whole genome sequences of all 13 strains analysed in this study (in red) were examined alongside those of 150 published MRLM strains, , downloaded from the NCBI database. The following MSSA genome sequences were also included: seven reference strains <t>ATCC</t> <t>25923</t> <t>(ST243/CC30,</t> <t>CP009361.1),</t> ATCC 29213 (ST5, GCA_001267715.2), ATCC 12600 (ST8, GCA_006094915.1), ATCC 9144 (ST30, GCA_900457695.1), NCTC 8325 (ST8, GCA_000013425.1), MSSA476 (ST1, GCA_000011525.1) and MSSA6850 (ST50, GCA_000462955.1), and eight previously reported clinical MSSA strains 10-00598 (ST22, GCA_028076695.1), 15-02736 (ST291, GCA_028082935.1), 16-01080 (ST45, GCA_028080235.1), 16-02665 (ST25, GCA_028075835.1), 18-01695 (ST398, GCA_028081975.1), 18-01787 (ST5, GCA_028081295.1), 19-00515 (ST15, GCA_028081935.1), and C00012754 (ST20, ERR418969). , Sequence types (STs) (inner ring), clonal complexes (CCs) (middle ring) and spa types (outer ring) are annotated. The tree scale indicates the number of substitutions per genome.
    Mssa Strain Atcc 25923, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A single-nucleotide polymorphism-based phylogenetic tree of methicillin resistance-lacking mec (MRLM) strains of Staphylococcus aureus . The whole genome sequences of all 13 strains analysed in this study (in red) were examined alongside those of 150 published MRLM strains, , downloaded from the NCBI database. The following MSSA genome sequences were also included: seven reference strains ATCC 25923 (ST243/CC30, CP009361.1), ATCC 29213 (ST5, GCA_001267715.2), ATCC 12600 (ST8, GCA_006094915.1), ATCC 9144 (ST30, GCA_900457695.1), NCTC 8325 (ST8, GCA_000013425.1), MSSA476 (ST1, GCA_000011525.1) and MSSA6850 (ST50, GCA_000462955.1), and eight previously reported clinical MSSA strains 10-00598 (ST22, GCA_028076695.1), 15-02736 (ST291, GCA_028082935.1), 16-01080 (ST45, GCA_028080235.1), 16-02665 (ST25, GCA_028075835.1), 18-01695 (ST398, GCA_028081975.1), 18-01787 (ST5, GCA_028081295.1), 19-00515 (ST15, GCA_028081935.1), and C00012754 (ST20, ERR418969). , Sequence types (STs) (inner ring), clonal complexes (CCs) (middle ring) and spa types (outer ring) are annotated. The tree scale indicates the number of substitutions per genome.

    Journal: JAC-Antimicrobial Resistance

    Article Title: Genetic and clinical landscape of methicillin-resistant Staphylococcus aureus isolates lacking the mec gene, in Japan

    doi: 10.1093/jacamr/dlag008

    Figure Lengend Snippet: A single-nucleotide polymorphism-based phylogenetic tree of methicillin resistance-lacking mec (MRLM) strains of Staphylococcus aureus . The whole genome sequences of all 13 strains analysed in this study (in red) were examined alongside those of 150 published MRLM strains, , downloaded from the NCBI database. The following MSSA genome sequences were also included: seven reference strains ATCC 25923 (ST243/CC30, CP009361.1), ATCC 29213 (ST5, GCA_001267715.2), ATCC 12600 (ST8, GCA_006094915.1), ATCC 9144 (ST30, GCA_900457695.1), NCTC 8325 (ST8, GCA_000013425.1), MSSA476 (ST1, GCA_000011525.1) and MSSA6850 (ST50, GCA_000462955.1), and eight previously reported clinical MSSA strains 10-00598 (ST22, GCA_028076695.1), 15-02736 (ST291, GCA_028082935.1), 16-01080 (ST45, GCA_028080235.1), 16-02665 (ST25, GCA_028075835.1), 18-01695 (ST398, GCA_028081975.1), 18-01787 (ST5, GCA_028081295.1), 19-00515 (ST15, GCA_028081935.1), and C00012754 (ST20, ERR418969). , Sequence types (STs) (inner ring), clonal complexes (CCs) (middle ring) and spa types (outer ring) are annotated. The tree scale indicates the number of substitutions per genome.

    Article Snippet: A comparative analysis of the nucleotide sequences or deduced amino acid sequences extracted from 13 MRLMs with those from the MSSA reference strain ATCC 25923 (ST243/CC30, CP009361.1) was conducted to identify mutations in the pbp4 promoter and amino acid substitutions in PBPs 1-4, GdpP, YjbH and AcrB.

    Techniques: Sequencing